Recombinase Polymerase Amplification:Article Title: An age-related decrease in leptin contributes to CD8+ T cell aging in the tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse CD8a (Clone 53-6.7) eBioscience Cat# 25-0081-82; RRID: AB_469584 Anti-mouse-IFN gamma (Clone XMG1.2) eBioscience Cat# 17-7311-82; RRID: AB_469504 Anti-mouse-TNF alpha (Clone MP6-XT22) eBioscience Cat# 12-7321-82; RRID: AB_466199 Anti-human CD103 (Clone Ber-ACT8) eBioscience Cat# 11-1037-41; RRID: AB_1659665 Anti-human CD39 (Clone eBioA1(A1)) eBioscience Cat# 17-0399-42; RRID: AB_1963578 Anti-human CD57 (Clone TB01(TBO1)) eBioscience Cat# 48-0577-42; RRID: AB_2016680 Anti-human CD314 (Clone 1D11) eBioscience Cat# 11-5878-42; RRID: AB_2572509 Anti-human CD28 (Clone CD28.2) eBioscience Cat# 11-0289-42; RRID: AB_10733610 Anti-mouse-CD3e (Clone 145-2C11) eBioscience Cat# 17-0031-83; RRID: AB_469316 Anti-mouse-Ki-67 (Clone SolA15) eBioscience Cat# 12-5698-82; RRID: AB_11150954 Anti-mouse CD366 (TIM3) (Clone RMT3-23) eBioscience Cat# 25-5870-82; RRID: AB_2573483 Anti-mouse 45 (Clone 30-F11) eBioscience Cat# 17-0451-82; RRID: AB_469392 Anti-mouse CD279 (PD-1) (Clone 29F.1A12) BioLegend Cat# 135213; RRID: AB_10689633 Anti-human CD8a (Clone RPA-T8) eBioscience Cat# 17-0088-42; RRID: AB_10669564 P16 INK4A (Clone D7C1M) Rabbit mAb Cell Signaling Technology Cat# 82548; RRID: AB_2799995 p-p38 (Thr180/Tyr182) (Clone 28B10) mAb Cell Signaling Technology Cat# 4552; RRID: AB_331303 Anti-CDKN2A/p16INK4a Abcam Cat# ab211542; RRID: AB_2891084 Goat anti-Rabbit IgG Second Antibody, Alexa FluorTM 488 Invitrogen Cat# A11034; RRID: AB_2541675 Anti-H2A.X Phospho (Ser 139) (Clone 2F3) BioLegend Cat# 613420; RRID: AB_2715785 Anti-GFP Abcam Cat# ab6556; RRID: AB_305564 Anti-mouse CD3e (Clone 145-2C11) ThermoFisher Cat# 16-0031-86; RRID: AB_468849 Anti-mouse CD28 (Clone 37.51) ThermoFisher Cat# 16-0281-86; RRID: AB_468923 InVivoMAb anti-mouse PD-1 (RMP1-14) Bio X cell Cat# BE0146; RRID: AB_10949053 InVivoMAb rat IgG2b isotype Control (2A3) Bio X cell Cat# BE0089; RRID: AB_1107769 (Continued on next page) e1 Cell Reports Medicine 6, 102310, September 16, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER InVivoMAb anti-mouse CD8α (2.43) Bio X cell Cat# BE0061; RRID: AB_1125541 InVivoMAb rat IgG2b isotype Control (LTF-2) Bio X cell Cat# BE0090; RRID: AB_1107780 Biological samples Human colorectal cancer (CRC) tissues Ruijin Hospital N/A Human colorectal cancer (CRC) patientderived blood Ruijin Hospital N/A Human lung cancer (LC) tissues Shanghai Chest Hospital N/A Human lung cancer (LC) patientderived blood Shanghai Chest Hospital N/A Human renal cell carcinoma (RCC) tissues Xinhua Hospital N/A Human renal cell carcinoma (RCC) patientderived blood Xinhua Hospital N/A Human renal cell carcinoma (RCC) paratumor tissues Xinhua Hospital N/A Chemicals, peptides, and recombinant proteins Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# P8139 Ionomycin Sigma-Aldrich Cat# I3909 Monensin eBioscience Cat# 65-4505-51 Fixation/Permeabilization Concentrate eBioscience Cat# 00-5123-43 Fixation/Permeabilization Diluent eBioscience Cat# 00-5233-56 Permeabilization buffer 10× eBioscience Cat# 00-8333-56 Protease Inhibitor MedChem Express Cat# HY-K0010 Phosphatase Inhibitor MedChem Express Cat# HY-K0021 DNase I Roche Cat# 10104159001 Collagenase IV Sigma-Aldrich Cat# C5138 Collagenase VIII Sigma-Aldrich Cat# C2139 Percoll GE Healthcare Cat# 17-0891-09 Ficoll-Paque Plus GE Healthcare Cat# 17-1440-03 C12FDG MedChem Express Cat# HY-126839 Bafilomycin A1 Selleck Cat# S1413 Dasatinib Selleck Cat# S1021 Quercetin Sigma Aldrich Cat# Q4951 Phosal 50 PG MedChem Express Cat# HY-Y1903 PEG-400 Sigma Aldrich Cat# 91893 Tween-80 Aladdin Cat# T104865 Leptin R&D Systems Cat# 498.OB Orlistat MedChem Express Cat# HY-B0218 Ovalbumin (257–264) chicken Sigma-Aldrich Cat# S7951 TRIzol Reagent Invitrogen Cat# 15596018 Recombinant Mouse IL-2 R&D systems Cat# 402-ML/CF BODIPYTM 493/503 Invitrogen Cat# D3922 BODIPYTM FL C12 Invitrogen Cat# D3822 NBD cholesterol Invitrogen Cat# N1148 HLA-A*02:01 CMV pp65 TetramerNLVPMVATV MBL Cat# TS-0010 HLA-A*02:01 EBV LMP2 TetramerFLYALALLL MBL Cat# TS-M069 HLA-A*02:01 Influenza M1 TetramerGILGFVFTL MBL Cat# TS-0012 (Continued on next page) Cell Reports Medicine 6, 102310, September 16, 2025 e2 Article ll OPEN ACCESS
Control:Article Title: An age-related decrease in leptin contributes to CD8+ T cell aging in the tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse CD8a (Clone 53-6.7) eBioscience Cat# 25-0081-82; RRID: AB_469584 Anti-mouse-IFN gamma (Clone XMG1.2) eBioscience Cat# 17-7311-82; RRID: AB_469504 Anti-mouse-TNF alpha (Clone MP6-XT22) eBioscience Cat# 12-7321-82; RRID: AB_466199 Anti-human CD103 (Clone Ber-ACT8) eBioscience Cat# 11-1037-41; RRID: AB_1659665 Anti-human CD39 (Clone eBioA1(A1)) eBioscience Cat# 17-0399-42; RRID: AB_1963578 Anti-human CD57 (Clone TB01(TBO1)) eBioscience Cat# 48-0577-42; RRID: AB_2016680 Anti-human CD314 (Clone 1D11) eBioscience Cat# 11-5878-42; RRID: AB_2572509 Anti-human CD28 (Clone CD28.2) eBioscience Cat# 11-0289-42; RRID: AB_10733610 Anti-mouse-CD3e (Clone 145-2C11) eBioscience Cat# 17-0031-83; RRID: AB_469316 Anti-mouse-Ki-67 (Clone SolA15) eBioscience Cat# 12-5698-82; RRID: AB_11150954 Anti-mouse CD366 (TIM3) (Clone RMT3-23) eBioscience Cat# 25-5870-82; RRID: AB_2573483 Anti-mouse 45 (Clone 30-F11) eBioscience Cat# 17-0451-82; RRID: AB_469392 Anti-mouse CD279 (PD-1) (Clone 29F.1A12) BioLegend Cat# 135213; RRID: AB_10689633 Anti-human CD8a (Clone RPA-T8) eBioscience Cat# 17-0088-42; RRID: AB_10669564 P16 INK4A (Clone D7C1M) Rabbit mAb Cell Signaling Technology Cat# 82548; RRID: AB_2799995 p-p38 (Thr180/Tyr182) (Clone 28B10) mAb Cell Signaling Technology Cat# 4552; RRID: AB_331303 Anti-CDKN2A/p16INK4a Abcam Cat# ab211542; RRID: AB_2891084 Goat anti-Rabbit IgG Second Antibody, Alexa FluorTM 488 Invitrogen Cat# A11034; RRID: AB_2541675 Anti-H2A.X Phospho (Ser 139) (Clone 2F3) BioLegend Cat# 613420; RRID: AB_2715785 Anti-GFP Abcam Cat# ab6556; RRID: AB_305564 Anti-mouse CD3e (Clone 145-2C11) ThermoFisher Cat# 16-0031-86; RRID: AB_468849 Anti-mouse CD28 (Clone 37.51) ThermoFisher Cat# 16-0281-86; RRID: AB_468923 InVivoMAb anti-mouse PD-1 (RMP1-14) Bio X cell Cat# BE0146; RRID: AB_10949053 InVivoMAb rat IgG2b isotype Control (2A3) Bio X cell Cat# BE0089; RRID: AB_1107769 (Continued on next page) e1 Cell Reports Medicine 6, 102310, September 16, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER InVivoMAb anti-mouse CD8α (2.43) Bio X cell Cat# BE0061; RRID: AB_1125541 InVivoMAb rat IgG2b isotype Control (LTF-2) Bio X cell Cat# BE0090; RRID: AB_1107780 Biological samples Human colorectal cancer (CRC) tissues Ruijin Hospital N/A Human colorectal cancer (CRC) patientderived blood Ruijin Hospital N/A Human lung cancer (LC) tissues Shanghai Chest Hospital N/A Human lung cancer (LC) patientderived blood Shanghai Chest Hospital N/A Human renal cell carcinoma (RCC) tissues Xinhua Hospital N/A Human renal cell carcinoma (RCC) patientderived blood Xinhua Hospital N/A Human renal cell carcinoma (RCC) paratumor tissues Xinhua Hospital N/A Chemicals, peptides, and recombinant proteins Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# P8139 Ionomycin Sigma-Aldrich Cat# I3909 Monensin eBioscience Cat# 65-4505-51 Fixation/Permeabilization Concentrate eBioscience Cat# 00-5123-43 Fixation/Permeabilization Diluent eBioscience Cat# 00-5233-56 Permeabilization buffer 10× eBioscience Cat# 00-8333-56 Protease Inhibitor MedChem Express Cat# HY-K0010 Phosphatase Inhibitor MedChem Express Cat# HY-K0021 DNase I Roche Cat# 10104159001 Collagenase IV Sigma-Aldrich Cat# C5138 Collagenase VIII Sigma-Aldrich Cat# C2139 Percoll GE Healthcare Cat# 17-0891-09 Ficoll-Paque Plus GE Healthcare Cat# 17-1440-03 C12FDG MedChem Express Cat# HY-126839 Bafilomycin A1 Selleck Cat# S1413 Dasatinib Selleck Cat# S1021 Quercetin Sigma Aldrich Cat# Q4951 Phosal 50 PG MedChem Express Cat# HY-Y1903 PEG-400 Sigma Aldrich Cat# 91893 Tween-80 Aladdin Cat# T104865 Leptin R&D Systems Cat# 498.OB Orlistat MedChem Express Cat# HY-B0218 Ovalbumin (257–264) chicken Sigma-Aldrich Cat# S7951 TRIzol Reagent Invitrogen Cat# 15596018 Recombinant Mouse IL-2 R&D systems Cat# 402-ML/CF BODIPYTM 493/503 Invitrogen Cat# D3922 BODIPYTM FL C12 Invitrogen Cat# D3822 NBD cholesterol Invitrogen Cat# N1148 HLA-A*02:01 CMV pp65 TetramerNLVPMVATV MBL Cat# TS-0010 HLA-A*02:01 EBV LMP2 TetramerFLYALALLL MBL Cat# TS-M069 HLA-A*02:01 Influenza M1 TetramerGILGFVFTL MBL Cat# TS-0012 (Continued on next page) Cell Reports Medicine 6, 102310, September 16, 2025 e2 Article ll OPEN ACCESS
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